Visualising statistical significance thresholds on EEG data

MNE-Python provides a range of tools for statistical hypothesis testing and the visualisation of the results. Here, we show a few options for exploratory and confirmatory tests - e.g., targeted t-tests, cluster-based permutation approaches (here with Threshold-Free Cluster Enhancement); and how to visualise the results.

The underlying data comes from [1]; we contrast long vs. short words. TFCE is described in [2].


[1]Dufau, S., Grainger, J., Midgley, KJ., Holcomb, PJ. A thousand words are worth a picture: Snapshots of printed-word processing in an event-related potential megastudy. Psychological Science, 2015
[2]Smith and Nichols 2009, “Threshold-free cluster enhancement: addressing problems of smoothing, threshold dependence, and localisation in cluster inference”, NeuroImage 44 (2009) 83-98.
import numpy as np
import matplotlib.pyplot as plt
from scipy.stats import ttest_ind

import mne
from mne.channels import find_ch_connectivity, make_1020_channel_selections
from mne.stats import spatio_temporal_cluster_test


# Load the data
path = mne.datasets.kiloword.data_path() + '/kword_metadata-epo.fif'
epochs = mne.read_epochs(path)
name = "NumberOfLetters"

# Split up the data by the median length in letters via the attached metadata
median_value = str(epochs.metadata[name].median())
long_words = epochs[name + " > " + median_value]
short_words = epochs[name + " < " + median_value]


Reading /home/circleci/mne_data/MNE-kiloword-data/kword_metadata-epo.fif ...
Isotrak not found
    Found the data of interest:
        t =    -100.00 ...     920.00 ms
        0 CTF compensation matrices available
960 matching events found
No baseline correction applied
960 matching events found
No baseline correction applied
Adding metadata with 8 columns
0 projection items activated

If we have a specific point in space and time we wish to test, it can be convenient to convert the data into Pandas Dataframe format. In this case, the mne.Epochs object has a convenient mne.Epochs.to_data_frame() method, which returns a dataframe. This dataframe can then be queried for specific time windows and sensors. The extracted data can be submitted to standard statistical tests. Here, we conduct t-tests on the difference between long and short words.

time_windows = ((.2, .25), (.35, .45))
elecs = ["Fz", "Cz", "Pz"]

# display the EEG data in Pandas format (first 5 rows)

report = "{elec}, time: {tmin}-{tmax} s; t({df})={t_val:.3f}, p={p:.3f}"
print("\nTargeted statistical test results:")
for (tmin, tmax) in time_windows:
    long_df = long_words.copy().crop(tmin, tmax).to_data_frame()
    short_df = short_words.copy().crop(tmin, tmax).to_data_frame()
    for elec in elecs:
        # extract data
        A = long_df[elec].groupby("condition").mean()
        B = short_df[elec].groupby("condition").mean()

        # conduct t test
        t, p = ttest_ind(A, B)

        # display results
        format_dict = dict(elec=elec, tmin=tmin, tmax=tmax,
                           df=len( - 2, t_val=t, p=p)


Converting time column to int64...
signal                      Fz  ...        Pz
condition epoch time            ...
film      0     -100  0.421970  ...  0.398182
                -96   0.453939  ...  0.222424
                -92   0.465606  ...  0.018485
                -88   0.468182  ... -0.173485
                -84   0.483485  ... -0.312121

[5 rows x 3 columns]

Targeted statistical test results:
Converting time column to int64...
Converting time column to int64...
Fz, time: 0.2-0.25 s; t(958)=-0.572, p=0.568
Cz, time: 0.2-0.25 s; t(958)=-2.836, p=0.005
Pz, time: 0.2-0.25 s; t(958)=-3.938, p=0.000
Converting time column to int64...
Converting time column to int64...
Fz, time: 0.35-0.45 s; t(958)=5.192, p=0.000
Cz, time: 0.35-0.45 s; t(958)=5.555, p=0.000
Pz, time: 0.35-0.45 s; t(958)=6.353, p=0.000

Absent specific hypotheses, we can also conduct an exploratory mass-univariate analysis at all sensors and time points. This requires correcting for multiple tests. MNE offers various methods for this; amongst them, cluster-based permutation methods allow deriving power from the spatio-temoral correlation structure of the data. Here, we use TFCE.

# Calculate statistical thresholds
con = find_ch_connectivity(, "eeg")

# Extract data: transpose because the cluster test requires channels to be last
# In this case, inference is done over items. In the same manner, we could
# also conduct the test over, e.g., subjects.
X = [long_words.get_data().transpose(0, 2, 1),
     short_words.get_data().transpose(0, 2, 1)]
tfce = dict(start=.2, step=.2)

t_obs, clusters, cluster_pv, h0 = spatio_temporal_cluster_test(
    X, tfce, n_permutations=100)  # a more standard number would be 1000+
significant_points = cluster_pv.reshape(t_obs.shape).T < .05
print(str(significant_points.sum()) + " points selected by TFCE ...")


Could not find a connectivity matrix for the data. Computing connectivity based on Delaunay triangulations.
-- number of connected vertices : 29
stat_fun(H1): min=0.000000 max=81.298503
Running initial clustering
Using 406 thresholds from 0.20 to 81.20 for TFCE computation (h_power=2.00, e_power=0.50)
Found 7424 clusters
Permuting 99 times...

Computing cluster p-values
1461 points selected by TFCE ...

The results of these mass univariate analyses can be visualised by plotting mne.Evoked objects as images (via mne.Evoked.plot_image) and masking points for significance. Here, we group channels by Regions of Interest to facilitate localising effects on the head.

# We need an evoked object to plot the image to be masked
evoked = mne.combine_evoked([long_words.average(), -short_words.average()],
                            weights='equal')  # calculate difference wave
time_unit = dict(time_unit="s")
evoked.plot_joint(title="Long vs. short words", ts_args=time_unit,
                  topomap_args=time_unit)  # show difference wave

# Create ROIs by checking channel labels
selections = make_1020_channel_selections(, midline="12z")

# Visualize the results
fig, axes = plt.subplots(nrows=3, figsize=(8, 8))
axes = {sel: ax for sel, ax in zip(selections, axes.ravel())}
evoked.plot_image(axes=axes, group_by=selections, colorbar=False, show=False,
                  mask=significant_points, show_names="all", titles=None,
plt.colorbar(axes["Left"].images[-1], ax=list(axes.values()), shrink=.3,
  • ../_images/sphx_glr_plot_stats_cluster_erp_001.png
  • ../_images/sphx_glr_plot_stats_cluster_erp_002.png

Total running time of the script: ( 0 minutes 28.755 seconds)

Estimated memory usage: 65 MB

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